LIFE SCIENCE LABORATORY
Central Research Laboratory - Basic and Applied Sciences
This is an interdisciplinary laboratory which is working in various research areas in biological sciences including biotechnology and molecular biology. Students can pursue M.Sc. by research and Ph.D. in Zoology through the laboratory. We are planning to get recognition for the laboratory in Applied Biology.
Our research is funded through grants from private trusts and Government of India bodies such as Department of Ocean Development (DOD), Department of Science and Technology (DST), Department of Biotechnology (DBT) and University Grants Commission (UGC). We also provide consultancy services for Environment Impact Assessments (EIA), biodiversity and biomedical research. We also provide short-term training in micro-technique, animal tissue culture and microbiology techniques. Further we are also planning to start with short term techniques in Molecular Biology. We have been working on two important areas of research in biotechnology and biological sciences.
Our principal research work is in the area of Marine Biotechnology. We started with Marine biotechnology research as a small group of M.Sc. students and one Lecturer from the College in 1990. We are now recognized as one of the foremost group of researchers actively involved in the field of marine Biotechnology in India as well as abroad.
In marine biotechnology, our interest is in marine bioactive compounds and harnessing biofuels from marine sources. The laboratory has well-equipped animal tissue culture facility which is a feather in our cap. Antibacterial, immunomodulatory, cytotoxicity and anti-oxidant activity are some of the ongoing activities in our laboratory. We also work on mechanism of action of marine and herbal bioactive compound on osteoporosis, process of angiogenesis and tubulin binding capacity.
We are also working on a project to study the potential of extracting biofuel from a novel marine micro-alga. It has been deposited in NCBI gene bank with accession number KR871012. The Laboratory has successfully established and standardized a lab-scale micro-alga culture yielding promising amounts of total lipids. Our future work on KR871012 is to establish it as new species and to standardize the technology for large scale culture set up.
In our second priority area of research, ecology and biodiversity, some students are working on biodiversity studies of spiders, butterflies and birds. Under chemical ecology, a doctoral student is working on pheromones of lepidopteran butterfly and its behavioral aspect. Another student is working on taxonomy and biodiversity of foraminifera, a group of marine protozoans.
The lab is self-sufficient and the revenue generated through consultancy and workshops is utilized for purchasing chemicals, glassware and maintenance of equipments. The entire work of washing, cleaning of glassware, cleanliness of laboratory and most importantly; the sterile condition of Animal Tissue Culture Laboratory and it is maintained by students. The good practices in the laboratory are as follows:
- Journal club – every week one student makes a presentation on a recently published paper from a good impact factor journal related to his/her research topic.
- Every month students report the work.
- Students co-operate in their experimental work resulting in a healthy and cordial environment in the laboratory.
- Research students keep records of all registers.
- Students keep account of finances.
- Students are trained to write projects and their budgets.
Prepared by:
Dr. Madhavi Indap
Prof. Emeritus, Adjunct faculty.
Projects completed
In last few years we have continuatively completed following projects of various funding agencies successfully and published about 25 papers and 10 Ph. D. degrees
|
2011-2013 |
UGC Emeritus fellowship project |
|
2008-2011
|
Charaterisation of bioactive compounds from marine mollusc and porifera that regulate osteoclastogenesis, Department of Biotechnology, Ministry of Science and Technology, Government of India. This project is in collaboration with Prof. Dr. Shubhada Chiplunkar, Director and Head Immunology, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Kharghar, |
|
2007-2010
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Modulatory effect of extracts of different marine organisms on drug-induced clastogenicity, University Grant Commission (UGC). |
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2006-2007
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Immunomodulatory and antioxidant activities of extracts from marine organisms, R. D. Birla Smarak Kosh, Medical Research Centre, Bombay Hospital Trust, Mumbai. |
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2004-2007
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Isolation, purification and stabilization of tubulin, Department of Science and Technology (DST), Government of India. This project is in collaboration with Dr. Arvind Lali, Department of Chemical Engineering. Institute of Chemical Technology (ICT), Mumbai. |
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2000-2003
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Effect of petroleum film on ecology of phytoplankton along the west coast of Mumbai. Department of Ocean Development (DOD), Government of India. |
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2002-2004
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Evaluation of some marine animal extracts for immunomodulatory activities, University Grants Commission (UGC). |
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1999 2000
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Study of marine animal extracts as angiogenesis inhibitor and its probable use as antifertility substance, University of Mumbai, |
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1997-1999
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Study of natural substances of marine origin: their structure and bioactivity, University Grants Commission (UGC). |
Project sanctioned
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2014
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D.B.T project entitled, ‘Investigation of chemical nature of bioactive compounds from the active fractions of Turbo brunneus extract and to study their mode of action in regulation of osteoclastogenesis.’ Ref.no. BT/45/11563, 50 lacs |
Dr. Rajan V. Rele
List of publications till date
|
Sr. No |
Title |
Journal |
ISSN/ISBNNo. |
Whether Peer reviewed Impact factor, if any |
No. of co-authors |
Whether you are main author |
|
1. |
Advance simultaneous determination of amiloride hydrochloride, hydrochlorothiazide and Atenolol in capsules by RP- HPLC 46(3), 231-236,2009.
|
Indian Drugs. |
ISSN: 0019-462X) |
Yes (0.4) |
2 |
yes |
|
2. |
Simultaneous determination of Clotrimazole, Beclomethasone, methyl paraben, propyl paraben in creams by RP- HPLC., 46(4),323-328, 2009. |
Indian Drugs |
(ISSN: 0019-462X) |
Yes (0.4) |
2 |
yes |
|
3. |
Simple extractive spectrophotometric determination of desloratadine from pharmaceutical formulation. 7(3), 1680-1686, 2009 . |
International Journal of chemical Sciences |
ISSN:0922-768X)
|
Yes |
2 |
Yes |
|
4. |
Advance Reverse Phase High Pressure Liquid Chromatographic method for determination of desloratadine from pharmaceutical formulation, 7(4) , 2883-2890, 2009.
|
International Journal of chemical Sciences |
ISSN:0922-768X) |
Yes |
2 |
Yes |
|
5.
|
Simultaneous determination of aceclofanac, paracetamol and tizanidine in tablets Analytical RP-HPLC 8(2),161-164,2009. |
Chemistry: An Indian journal.
|
(ISSN: 0974-7419)
|
Yes
|
2
|
Yes
|
|
6. |
Simple spectrophotometric determination of desloratadine from pharmaceutical formulation. 8(2) 233-236, 2009 |
Analytical chemistry: An Indian Journal |
.(ISSN: 0974-7419) |
Yes |
2 |
Yes |
|
7. |
A validated non-aqueous potentiometric titration method for the quantitative determination of rupatadine as rupatadine fumarate. 8(2), 165-167, 2009. |
Analytical chemistry: An Indian Journal. |
(ISSN: 0974-7419) |
Yes |
2 |
Yes |
|
44 |
Simultaneous spectrophotometric estimation of amoxicillin and carbocisteine by first order derivative spectroscopy method in combined dosage form. |
Asian J. Research chem.. |
ISSN : 0974-4169 |
Yes |
- |
Yes |
|
|
45 |
Spectrophotometric Estimation of Ambroxal hydrochloride in Bulk Drug and Pharmaceutical Dosage Form by Area Under Curve Method. |
Research Journal Pham. and Tech. |
ISSN: 0974-3618 |
Yes |
- |
Yes
|
|
|
46 |
Ultra-Violet Spectrophotometric Method For Validation of Rupatadine Fumarate From Bulk Drug And Pharmaceutical Formulation |
American Journal of Pharm-Tech Research, |
ISSN: 2249-3387 |
Yes0.9832 |
- |
Yes |
|
|
47 |
UV Spectrophotometric Estimation of Rupatadine Fumarate by First Order Derivative and Area Under Curve Methods in Bulk and Pharmaceutical Dosage Form. |
Der pharmacia letter |
ISSN0975-5071.USA CODEN:DPLFB4 |
Yes(5.053) |
|
|
|
|
48 |
Simultaneous UV-Spectrophotometric Estimation of Amoxicillin and Carbocisteine by Area Under Curve Method in Combined Dosage Form. |
Der pharmacia lettre |
ISSN0975-5071.USA CODEN:DPLFB4 |
Yes(5.053) |
2 |
yes |
|
|
49 |
Simultaneous UV spectrophotometric determination of ambroxal hydrochloride and guaiphenesin by First order derivative method in combined dosage form |
Journal of chemical and pharmaceutical research, |
( ISSN: 0975-7384) CODEN(USA):JCPRC5 |
Yes(0.92) |
|
|
|
|
50
|
Simultaneous UV spectrophotometric determination of ambroxal hydrochloride and guaiphenesin by area under curve method in combined dosage form , |
Research Journal Pham. and Tech. |
ISSN: 0974-3618 |
Yes |
- |
Yes |
|
|
51 |
Simultaneous UV spectrophotometric determination of ambroxal hydrochloride and guaiphenesin by second order derivative method in combined dosage form |
Asian J. Research chem.. |
ISSN : 0974-4169 |
Yes |
- |
yes |
|
|
52 |
Simultaneous Spectrophotometric Estimation of Ofloxacin and Ornidazole by second Order Derivative Spectroscopy Method in Combined Dosage |
Der pharma lettre |
ISSN0975-5071.USA CODEN:DPLFB4 |
Yes(5.053) |
|
yes |
|
|
53 |
Spectrophotometric Estimation of Azelnidipine in Bulk and Pharmaceutical Dosage Form by second Order Derivative Method. |
Journal of chemical and pharmaceutical research, |
( ISSN: 0975-7384) CODEN(USA):JCPRC5 |
Yes(0.92) |
- |
yes |
|
|
54 |
Spectrophotometric estimation of oxolamine citrate in bulk and pharmaceutical dosage form by second order derivative method. |
Research Journal Pham. and Tech. |
ISSN: 0974-3618 |
yes |
- |
yes
|
|
|
55 |
Spectrophotometric estimation of carbocisteine in bulk and pharmaceutical dosage form by second order derivative method |
Journal of chemical and pharmaceutical research, |
( ISSN: 0975-7384) CODEN (USA):JCPRC5 |
Yes(0.92) |
- |
yes |
|
|
56 |
UV Spectrophotometric Estimation of Rupatadine Fumarate by second Order Derivative and Method in Bulk and Pharmaceutical Dosage Form |
Asian J.Research chemistry |
ISSN : 0974-4169 |
Yes |
- |
yes |
|
|
57 |
Simultaneous spectrophotometric estimation of amoxicillin and carbocisteine by second order derivative spectroscopy method in combined dosage form. |
international journal of chemical sciences |
ISSN:0922-768X) |
Yes |
|
yes |
|
|
58 |
Simultaneous spectrophotometric estimation of amoxicillin and bromhexine hydrochloride by second order derivative spectroscopy method in combined dosage form. |
International journal of chemical sciences |
(ISSN:0972-768X) |
Yes |
|
yes |
|
|
59 |
Simultaneous spectrophotometric estimation of amoxicillin trihydrate and ambroxal hydrochloride by second order derivative spectroscopy method in combined dosage form. |
Research Journal Pham. and Tech. |
ISSN: 0974-3618 |
yes |
-
|
yes
|
|
|
60 |
UV Spectrophotometric Estimation of ambroxal hydrochloride by second Order Derivative and Method in Bulk and Pharmaceutical Dosage Form. |
Journal of chemical and pharmaceutical research, |
( ISSN: 0975-7384) CODEN (USA):JCPRC5 |
Yes(0.92) |
- |
yes |
|
|
61 |
Validation Method of Oxolamine Citrate from Bulk Drug and Pharmaceutical Formulation by High Performance Liquid Chromatography Method |
international journal of pharmtech |
CODEN (USA): IJPRIF, ISSN: 0974-4304 |
Yes |
- |
yes |
|
|
62 |
Spectrophotometric Estimation of carvedilol hydrochloride in Bulk and Pharmaceutical Dosage Form by First Order Derivative and Area Under Curve Methods. |
Der pharmacia sinica |
ISSN:0976-8688 CODEN:(USA):PSHIBD |
Yes(0.527) |
- |
yes |
|
|
63 |
Spectrophotometric Estimation of carvedilol in Bulk and Pharmaceutical Dosage Form by second Order Derivative Method. |
Research Journal Pham. and Tech. |
ISSN: 0974-3618 |
Yes |
- |
yes
|
|
|
64 |
Spectrophotometric Estimation of Clopidogrel in Bulk and Pharmaceutical Dosage Form by second Order Derivative Method |
Der pharmacia lettre |
ISSN0975-5071.USA CODEN:DPLFB4 |
Yes(5.027) |
|
yes |
|
|
65 |
Advance Reversed Phase high Performance Liquid Chromatography Method for Determination of Clopidogrel bisulphate from Active Pharmaceutical dosage form |
Journal of chemical and pharmaceutical research, |
( ISSN: 0975-7384) CODEN(USA):JCPRC5 |
Yes(0.92) |
- |
yes |
|
|
66 |
Determination of Clopidogrel Bisulphate in Pharmaceutical Dosage Form by High Performance Liquid Chromatography Method, |
Der pharmacia sinica |
ISSN:0976-8688CODEN:(USA):PSHIBD |
Yes(0.527) |
- |
yes |
|
|
67 |
Reversed Phase high Performance Liquid Chromatography Method for Determination of olopatadine hydrochloride from Active Pharmaceutical dosage form, |
der pharmacia lettre |
ISSN0975-5071.USA CODEN:DPLFB4 |
Yes(5.027) |
|
yes |
|
|
68 |
Reversed Phase high Performance Liquid Chromatography Method for Determination of Carvedilol hydrochloride from Active Pharmaceutical dosage form, |
Journal of chemical and pharmaceutical research, |
( ISSN: 0975-7384) CODEN(USA):JCPRC5 |
Yes(0.92) |
- |
yes |
|
|
69 |
Spectrophotometric Estimation of Olopatadine hydrochloride in Bulk and Pharmaceutical Dosage Form by area under curve and second Order Derivative Method |
Research Journal Pham. and Tech. |
ISSN: 0974-3618 |
Yes |
|
yes |
|
|
70 |
High performance liquid chromatography for validation method of oxolamine citrate from bulk drug and pharmaceutical formulation,7(1), 865-869, 2015. |
Journal of chemical and pharmaceutical research, |
( ISSN: 0975-7384) CODEN(USA):JCPRC5 |
Yes(0.92) |
- |
yes |
|
|
71 |
Reversed Phase High Performance Liquid Chromatography Method for Determination of Ambroxal Hydrochloride from Active Pharmaceutical dosage form |
Der pharma sinica |
ISSN:0976-8688CODEN:(USA):PSHIBD |
Yes (0.527) |
- |
yes |
|
|
72 |
Simultaneous Determination of Ambroxal hydrochloride and Guaiphenesin in Pharmaceutical Dosage by Reverse Phase High Performance Liquid Chromatography |
Der pharmacia lettre |
ISSN0975-5071.USA CODEN:DPLFB4 |
Yes (5.027) |
|
yes |
|
|
73 |
Simultaneous Determination of Ambroxal hydrochloride and salbutamol sulphate in Pharmaceutical Dosage by Reverse Phase High Performance Liquid Chromatography |
Journal of chemical and pharmaceutical research, |
( ISSN: 0975-7384) CODEN(USA):JCPRC5 |
Yes(0.92) |
- |
yes |
|
|
74. |
Simultaneous spectrophotometric estimation of Ambroxal hydrochloride and salbutamol sulphate by first order derivative method in Pharmaceutical Dosage form. |
Asian J.Research chemistry |
ISSN : 0974-4169 |
Yes |
- |
yes |
|
|
75 |
UV- spectrophotometric –second order derivative method for Simultaneous Determination of Ambroxal hydrochloride and salbutamol sulphate in Pharmaceutical Dosage |
Research Journal Pham. and Tech. |
ISSN: 0974-3618 |
Yes |
- |
yes |
|
|
76 |
Simultaneous Determination of Bromhexine hydrochloride and Salbutamol Sulphate in Pharmaceutical Dosage by Reverse Phase High Performance Liquid Chromatography |
Der pharmacia lettre |
ISSN0975-5071.USA CODEN:DPLFB4 |
Yes(5.027) |
|
yes |
|
|
77 |
Simultaneous Determination of Ambroxal hydrochloride, Guaiphenesin and Salbutamol Sulphate in Pharmaceutical Dosage by Reverse Phase High Performance Liquid Chromatography |
Der pharmacia lettre |
ISSN0975-5071.USA CODEN:DPLFB4 |
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